Proteomic characterization of BV-2 N-glycoproteome. (A) Overlap between 2 approaches (blue circle: whole cell capturing; red circle: crude membrane fraction capturing). (B) Identification of N-glycosylation sites and N-glycoproteins in 16 replicates by 2 different approaches. Orange bars and green bars represent the number of N-glycosylation sites and N-glycoproteins, respectively. (C) Frequency of consensus sequence N-x-T and N-x-S in all experiments. (D) Distribution of singly and multiply N-glycosylated proteins.