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Table 4 Validation of microarray data using qPCR

From: De novo assembly, characterization and functional annotation of Senegalese sole (Solea senegalensis) and common sole (Solea solea) transcriptomes: integration in a database and design of a microarray

   

Microarray

qPCR

SoleaDBcode

Gene

Gene name

FC

p-value

FC

p-value

Unigene18736

Angiotensin I converting enzyme 2

ace2

4.5

<0.001

4.9

<0.05

Unigene49603

Angiotensinogen

agt

3.5

<0.01

4.7

<0.05

Unigene39473

Na-K-Cl cotransporter2

nkcc2

2.5

<0.01

3.13

<0.01

Unigene252320

Transferrin

tf

15.6

<0.001

10.5

<0.01

Unigene214993

Ferritin

fth

2.1

<0.01

2.3

<0.05

Unigene39196

Heat shock protein 90-alpha

hsp90aa

2.7

<0.01

2.3

<0.01

Unigene54412

Trypsinogen1a

try1

17.6

<0.001

12.0

<0.001

Unigene31826

Trypsinogen2

try2

4.7

<0.001

7.8

<0.05

Unigene53434

Chymotrypsinogen2

ctr2

7.2

<0.001

6.3

<0.05

Unigene52166

Elastase1

cela1

8.7

<0.001

7.8

<0.05

Unigene53593

Elastase4

cela4

7.1

<0.001

4.6

<0.05

Unigene54920

Complement component C3

c3

3.8

<0.05

34.0

<0.05

Unigene53521

Lysozyme g

lyg

2.5

<0.05

3.6

<0.05

Unigene219622

Thyroid stimulating hormone, beta

tshb

2.5

<0.05

4.6

<0.001

Unigene52404

Transaldolase

taldo

2.1

<0.05

2.5

<0.05

  1. Fold-changes (FC) and p-values obtained for target genes by microarray and qPCR are indicated. Moreover, the transcript code in the SoleaDB for S. senegalensis v3 transcriptome is also shown. For qPCR, data were normalized to those of gapdh2 and referred to the calibrator group (36 ppt 3 DPH).