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Table 4 Validation of microarray data using qPCR

From: De novo assembly, characterization and functional annotation of Senegalese sole (Solea senegalensis) and common sole (Solea solea) transcriptomes: integration in a database and design of a microarray

    Microarray qPCR
SoleaDBcode Gene Gene name FC p-value FC p-value
Unigene18736 Angiotensin I converting enzyme 2 ace2 4.5 <0.001 4.9 <0.05
Unigene49603 Angiotensinogen agt 3.5 <0.01 4.7 <0.05
Unigene39473 Na-K-Cl cotransporter2 nkcc2 2.5 <0.01 3.13 <0.01
Unigene252320 Transferrin tf 15.6 <0.001 10.5 <0.01
Unigene214993 Ferritin fth 2.1 <0.01 2.3 <0.05
Unigene39196 Heat shock protein 90-alpha hsp90aa 2.7 <0.01 2.3 <0.01
Unigene54412 Trypsinogen1a try1 17.6 <0.001 12.0 <0.001
Unigene31826 Trypsinogen2 try2 4.7 <0.001 7.8 <0.05
Unigene53434 Chymotrypsinogen2 ctr2 7.2 <0.001 6.3 <0.05
Unigene52166 Elastase1 cela1 8.7 <0.001 7.8 <0.05
Unigene53593 Elastase4 cela4 7.1 <0.001 4.6 <0.05
Unigene54920 Complement component C3 c3 3.8 <0.05 34.0 <0.05
Unigene53521 Lysozyme g lyg 2.5 <0.05 3.6 <0.05
Unigene219622 Thyroid stimulating hormone, beta tshb 2.5 <0.05 4.6 <0.001
Unigene52404 Transaldolase taldo 2.1 <0.05 2.5 <0.05
  1. Fold-changes (FC) and p-values obtained for target genes by microarray and qPCR are indicated. Moreover, the transcript code in the SoleaDB for S. senegalensis v3 transcriptome is also shown. For qPCR, data were normalized to those of gapdh2 and referred to the calibrator group (36 ppt 3 DPH).