Skip to main content

Table 2 RNA-seq mapping statistics for pre- and post-globin reduction samples

From: Increasing gene discovery and coverage using RNA-seq of globin RNA reduced porcine blood samples

Ā 

pre-Globin Reduction

post-Globin Reduction

Sample

RINa

Total readsb

Aligned readsb

Expressed genesc

HBA readsb

HBB readsb

RINa

Total readsb

Aligned readsb

Expressed genesc

HBA readsb

HBB readsb

1

7.8

23.66

22.45

11269

7.57

4.19

8.0

18.00

16.45

12175

2.35

1.60

2

7.7

19.73

18.57

11591

5.43

3.40

7.8

21.62

19.56

12736

2.16

1.29

3

7.6

26.12

24.62

11445

8.24

4.96

7.7

21.68

19.91

12246

3.69

2.17

4

7.9

23.78

22.29

11796

6.68

3.80

7.5

25.57

22.94

12942

1.93

1.28

5

7.5

23.67

22.38

10966

7.23

4.63

7.4

19.81

17.59

12409

0.29

0.26

6

6.8

24.58

22.79

10671

6.30

3.82

7.0

17.79

15.60

11613

0.26

0.17

7

6.8

23.53

21.42

11517

2.82

2.76

6.4

21.03

18.50

11736

0.34

0.20

8

6.5

22.01

20.18

11900

4.54

2.13

5.9

18.40

16.20

12203

0.08

0.06

9

5.0

45.16

41.89

11464

9.36

12.64

4.9

25.33

21.86

11834

0.28

0.55

10

4.7

39.39

36.09

11319

8.46

8.37

4.5

34.33

29.36

12214

0.17

0.27

11

4.2

46.71

42.13

11292

8.92

9.74

4.1

34.64

29.45

11680

0.31

0.74

12

3.7

44.94

41.68

10441

9.96

12.16

3.5

31.81

27.31

11322

0.30

0.48

  1. aRIN: RNA Integrity Number provided by Agilent 2100 bioanalyzer.
  2. bPaired read counts are given in millions.
  3. cGenes are considered expressed when read count >5.
  4. A total of 24 samples were loaded onto different lanes according to RIN measured after globin reduction (sample 1ā€“6: high RIN, sample 7ā€“8: moderate RIN and sample 9ā€“12: low RIN).