Table 4 The scheme of how to apply I2b-RAD approach

Application

Chromosome assembly

QTL mapping

Enzyme

BsaXI

BsaXI

AlfI

Adapter

Adapter1, adapter2

Adapter1, adapter2 with 5′-NNF-3′ overhangs

Adapter1, adapter2 with 5′-NF-3′ overhangs

Enzyme site percentage

100%

1/16^th

1/16^th

The count of samples in a single lane (Oryza sativa for instance)

SE50 of 24 samples; PE50 of 48 samples;

SE50 of 384 samples; PE50 of 768 samples;

SE50 of 768 samples; PE50 of 1536 samples;