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Table 5 Comparison of microarray and quantitative PCR (qPCR) results for selected transcripts, encoding immune receptors

From: Transcriptional responses of PBMC in psychosocially stressed animals indicate an alerting of the immune system in female but not in castrated male pigs

Gene symbol Comparison Microarray Real-time PCR # Correlation ##
   p-value q-value expression FC p-value expression FC coefficient
CCR1 Females <0.05 <0.25 HSF > LSF +2.60 <0.10 HSF = LSF +1.81 0.93***
  Males <0.05 >0.25 HSM = LSM +1.75 >0.10 HSM = LSM +1.23  
CD14 Females <0.05 <0.25 HSF > LSF +3.89 <0.05 HSF > LSF +1.88 0.87***
  Males <0.05 >0.25 HSM = LSM +2.53 >0.10 HSM = LSM +1.11  
TLR2 Females <0.01 <0.25 HSF > LSF +1.82 <0.05 HSF > LSF +1.34 0.87***
  Males <0.05 >0.25 HSM = LSM +1.44 >0.10 HSM = LSM +1.10  
TLR4 Females <0.05 <0.25 HSF > LSF +3.71 <0.05 HSF > LSF +1.82 0.88***
  Males <0.10 >0.25 HSM = LSM +1.85 >0.10 HSM = LSM +1.15  
TREM1 Females <0.01 <0.25 HSF > LSF +2.80 <0.01 HSF > LSF +3.51 0.90***
  Males <0.01 >0.25 HSM = LSM +2.18 <0.10 HSM = LSM +2.09  
  1. Significance level was set at p <0.05 and q <0.25; FC - Fold change; # Values were calculated by factorial normalization on RPL32, PPIA and HPRT1 expression values; ## correlation of normalized expression values was calculated by Spearman (*** p < 0.0001).