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Table 5 Comparison of microarray and quantitative PCR (qPCR) results for selected transcripts, encoding immune receptors

From: Transcriptional responses of PBMC in psychosocially stressed animals indicate an alerting of the immune system in female but not in castrated male pigs

Gene symbol

Comparison

Microarray

Real-time PCR #

Correlation ##

  

p-value

q-value

expression

FC

p-value

expression

FC

coefficient

CCR1

Females

<0.05

<0.25

HSF > LSF

+2.60

<0.10

HSF = LSF

+1.81

0.93***

 

Males

<0.05

>0.25

HSM = LSM

+1.75

>0.10

HSM = LSM

+1.23

 

CD14

Females

<0.05

<0.25

HSF > LSF

+3.89

<0.05

HSF > LSF

+1.88

0.87***

 

Males

<0.05

>0.25

HSM = LSM

+2.53

>0.10

HSM = LSM

+1.11

 

TLR2

Females

<0.01

<0.25

HSF > LSF

+1.82

<0.05

HSF > LSF

+1.34

0.87***

 

Males

<0.05

>0.25

HSM = LSM

+1.44

>0.10

HSM = LSM

+1.10

 

TLR4

Females

<0.05

<0.25

HSF > LSF

+3.71

<0.05

HSF > LSF

+1.82

0.88***

 

Males

<0.10

>0.25

HSM = LSM

+1.85

>0.10

HSM = LSM

+1.15

 

TREM1

Females

<0.01

<0.25

HSF > LSF

+2.80

<0.01

HSF > LSF

+3.51

0.90***

 

Males

<0.01

>0.25

HSM = LSM

+2.18

<0.10

HSM = LSM

+2.09

 
  1. Significance level was set at p <0.05 and q <0.25; FC - Fold change; # Values were calculated by factorial normalization on RPL32, PPIA and HPRT1 expression values; ## correlation of normalized expression values was calculated by Spearman (*** p < 0.0001).