The predicted phospholipase A2-like (PLA2) from T. bicarinatum . (A) Amino acid alignment of the sequences of PLA2-like from T. bicarinatum (Tb21409, Tb14525, Tb34851, Tb5926 and Tb23564) with PLA2 from Apis mellifera [GenBank: P00630], Bombus terrestriris [GenBank: P82971], S. invicta [GenBank: EFZ20207], Heloderma suspectum [GenBank: P80003], Hadrurus gertschi [GenBank: P0C8L9], Pandinus imperator [GenBank: P59888] and Anuroctonus phaiodactylus [GenBank: Q6PXP0]. Strictly conserved and less conserved cysteines are indicated by black and grey shading, respectively and the predicted signal peptide cleavage sites for T. bicarinatum PLA2-like is indicated by a solid green triangle. The underlined regions indicate the Ca2+ binding loop and the active site: solid red triangle, Ca2+ binding residues; *, active site residues. The numbering of the amino acid (Gly32, His48, Asp49 and Asp99) follows that of the PLA2 from bovine pancreas . (B) Phylogenetic relationships of PLA2 from venomous animals based on amino acid sequence alignment. Phylogeny has been performed using the maximum likelihood method implemented in the PhyML program at http://www.phylogeny.fr. Numbers at nodes indicate bootstrap supports based on 100 replicates. Protein codes are as described in Figure 7A.