Figure 4

Age-associated DNA methylation signature independent of tissue type. (A, B) Examples of age-associated CpG loci with linear (A) or nonlinear (B) relationships identified in integrated normal samples. (C) Venn diagrams showing the numbers of age-associated CpG loci with three regression models (linear and second- and third-degree nonlinear) in integrated normal, cancer, or all samples. (D) Venn diagram showing the number of age-associated CpG loci among integrated normal, cancer, and all samples. (E) Manhattan plot of age-associated CpG loci in integrated normal samples by chromosome. Hypermethylated CpG loci with age are shown with a –log (P-value) and hypomethylated loci are shown with a log (P-value). The most significant P-values among linear and nonlinear models were chosen. Significant loci are marked as green (hypermethylated) or blue (hypomethylated) dots. The numbers of significant age-associated CpG loci by chromosome. Bar plots of P-values with hypergeometric tests for the degrees of significance of the numbers of the loci. (F) Manhattan plot of age-associated CpG loci in integrated cancer samples by chromosome.