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Table 2 Summary of the adenine methylation on the secondary endosymbiont’s helicase gene

From: Adenine methylation may contribute to endosymbiont selection in a clonal aphid population

Primers   A6F3 & A6R2   A6F1 & A6R1   A6F2 & A6R2  
Phenotype *No. of aphids DpnI DpnII DpnI + DpnII DpnI DpnII DpnI + DpnII DpnI DpnII DpnI + DpnII
22°C ( Orange ) 6 (6)+++ (6)+++ (6)+++ (6)+++ (6)+++ (6)+++ (2)++(4)+ (6)+++ (2)++(4)+
8°C ( Green ) 6 (6)+++ (6)+++ (6)++ (3)++(2)+ (6)+++ (3)++(3)+ (3)++ (6)+++ (2)+
  1. *Number of individual Acyrthosiphon pisum aphids tested by PCR amplification. The amplicon intensity was categorized into three levels: +++, high; ++, medium; +low; -, no amplification.