Figure 1

Identification pipeline for funRNA. The identification pipeline for funRNA consists of two steps: i) defining domain profiles from protein sequences encoded by the reference sequences; and ii) scanning 1,440 proteomes with domain profiles for Argonaute, Dicer, and RdRP. In "Domain analysis", colored boxes indicate essential domains: blue, IPR003100 (Argonaute/Dicer protein, PAZ); red, IPR003165 (Stem cell self-renewal protein Piwi); purple, IPR005034 (Dicer double-stranded RNA-binding fold); green, IPR000999 (Ribonuclease III); orange, IPR001159 (Double-stranded RNA-binding); and gray, IPR007855 (RNA-dependent RNA polymerase, eukaryotic type). In addition, sequences collected from funRNA can be subjected to bioinformatics analysis on the funRNA website as well as in CFGP 2.0 by data exchange through the Favorite Browser.