Figure 9

HOXD-AS1 expression is regulated by PI3K signaling pathway. SH-SY5Y cells were induced to differentiate with 10 µM retinoic acid (RA) in the absence or presence of 10 µM LY294002 (inhibitor of PI3K), PD98059 (inhibitor of MEK1) and SB203580 (inhibitor of p38-MAPK). RNA was extracted in the time-course of 6, 12 and 24 hours and the fold change of induction of HOXD-AS1 was estimated by qRT-PCR relative quantification analysis. Each treatment for each time-point was performed in triplicate with standard deviation error bars shown as indicated.