Figure 4

The distribution of amino acids along the tunnel when ribosomes stall. The position-specific probabilities were calculated for the 31 positions in the tunnel (based on the USRs). Results are presented per organism based on an aggregate that merges all analyzed datasets of the organism (see details in the Methods section: Merging all datasets of the organism into one aggregate). The probabilities were standardized to have a mean of zero per amino acid. We defined a square to be red/green if the probability to observe the amino acid in the corresponding position is significantly higher/lower than other positions in the tunnel.