Measurement of the kinetics of TBP binding to the oligodeoxyribonucleotide atcgggccgctataagTggggcgggc corresponding to rs34104384 (minor allele). A: Dependences of reaction rates on ODN concentration. B: Electropherograms from which these curves were derived. TBP concentration was 0.3 nM in all experiments; the concentrations of ODN were as indicated in the TBP/TATA-associated isotherms. The ka and kd values were calculated from the electropherograms using the GraphPad Prism 5 software (http://graphpad-prism.software.informer.com/5.01).