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Table 1 EMSA-based analysis of the complex of TBP and one of oligodeoxyribonucleotides (ODNs) in vitro.

From: Obesity-related known and candidate SNP markers can significantly change affinity of TATA-binding protein for human gene promoters

Gene probe 32P-labeled synthetic ODN, 26 bp, double-stranded DNA ka, × 103 M-1s-1 kd, × 10-4 s-1 K*D, nM t1/2, min -ΔG Kcal/mol
LEP WT atcgggccgcTATAAgaggggcgggc 2.3 ± 0.6 1.8 ± 0.6 78 64 ± 13 9.7 ± 0.9
LEP -38a atcgggccacTATAAGaggggcgggc 3.5 ± 0.5 1.5 ± 0.2 43 77 ± 16 10.0 ± 1.0
LEP -30t atcgggccgcTATAAGtggggcgggc 11.0 ± 2.0 8.0 ± 1.0 73 14 ± 3 9.7 ± 1.0
LEP -35g atcgggccgcTgTAAGaggggcgggc 5.6 ± 0.8 13.0 ± 2 232 9 ± 1 9.0 ± 0.9
GCG WT agctggagagTATATAaaagcagtgc 70 ± 10 5 ± 1.0 8 23 ± 5 11.1 ± 1.0
GCG -41g agctggagagTgTATAaaagcagtgc 30 ± 10 6 ± 1.0 18 19 ± 4 10.5 ± 0.9
  1. Notes: For each TBP-ODN-complex, ka is the association rate constant, kd is the dissociation rate constant, half-life (t1/2) equals (ln2)/kd, and K*D = kd/ka is the apparent dissociation constant. TATA-like subsequence: uppercase letters; ΔG = -RTln(ka/kd) is a change in Gibbs free energy, where R = 1.38 × 10-23 JK-1 is Boltzmann's constant, T is temperature in degrees Kelvin; the substitution corresponding to the minor allele of each SNP is shown in boldface.