Figure 3
From: Efficacy of SSH PCR in isolating differentially expressed genes
Enrichment of ratio differentially expressed φx174 DNA Hae III by SSH PCR. Fixed amount of 1.0% φx174 Hae III DNA was added in tester and various amount of φx174 Hae III DNA in driver, which resulted in a serial five folds of dilutions. SSH PCR was carried out as described in Materials and Methods. Lane 1, φx174 Hae III DNA marker; lane 2, 1.0% of φx174 Hae III DNA in driver; lane 3, 0.2% of φx174 Hae III DNA in driver; lane 4, 0.04% of φx174 Hae III DNA in driver; lane 5, 0.008% of φx174 Hae III DNA in driver; lane 6, 0.0016% of φx174 Hae III DNA in driver in driver; lane 7, no φx174 Hae III DNA in driver driver.