PEVE genome organization (A) The structure of Ul and Us regions that constitute the PEVE genome. ORFs are specified by open arrows, and their orientations by the direction of the arrows. Arrow numbers signify the length of the putative proteins encoded by ORFs. Small dark arrows indicate flanking inverted repeats. Simple arrows specify the primers used for PCR analysis shown in (B). M – marker, 100 bp ladder (Gibco BRL). positions of primer used for PCR were as follow: pr1 (Us region) 286–305; pr2 (Us region) 1518–1541; pr3 (Ul1 region) 461–482; pr4 (Ul1 region) 5430–5452; pr5 (Ul1 region) 1765–1786; pr6 (Ul1 region) 2985–3005. (C) Putative PEVE genome variants.