Figure 7

Sequence alignment of the AAA protomers in midasin with those in other branches of AAA-ATPase family The alignment is limited to residues contained in the P-loop, Walker B, and sensor 1 motifs of AAA2 and AAA3 protomers of midasin from humans and yeast, together with the corresponding residues in the AAA1 protomer of cytoplasmic dynein [6], and in a variety of other AAA proteins. The positions and lengths of the P-loop, Walker B, and sensor 1 motifs are taken from the review of Neuwald et al [5]. The color used for each residue in the alignment indicates its similarity to that present at the corresponding position in yeast cytoplasmic dynein. Color key: Yellow/red, all AAA proteins shown have identical residue; blue/blue, identical to AAA1 of dynein; green/green, closely similar to AAA1 of dynein; green/white, moderately similar; gray/white, not similar.