Figure 3
From: Multigene family isoform profiling from blood cell lineages
Validation and cross-reactivity of degenerate sub-family centred primers. 40 cycle PCR reactions were set up using degenerate sub-family centred primers against both non-cognate and cognate (positive control, left hand gel (a)-(g) clonal template. Detectable cross-reaction is indicated (arrowhead). Gel images were scanned and processed using Adobe Photoshop Version 5.5.