DNase I treatment of template prevents amplification of 500 bp product. Series of PCR reactions were performed on varying concentrations of clonal template DNA. Mass of template DNA (ng) and DNase I treatment (+/-) is indicated above the lanes. DNA ladder was run as molecular weight standard with sizes indicated (bp). Treatment with DNase I as described in Materials and Methods prevented amplification of 500 bp product from 10 μg of DNA. Gel image was scanned and processed using Adobe Photoshop Version 5.5.