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Table 2 PCR assay results using primer sets designed for pXO2 ORF sequences.

From: DNA sequence conservation between the Bacillus anthracis pXO2 plasmid and genomic sequence from closely related bacteria

ORF Nucleotides Gene ID or similarity S2-8 3A DC-17 ALH konku HD571 F1-15 4342 43881 33679 AWO6
5 2240–3088 unidentified            X
7 3449–4057 unidentified            
9 5227–7158 sim. trsE, S. aureus           X X
10 7178–7846 unidentified            X
14 8704–11562 c.h.p. C. perfringens            X
15 11588–12379 c.h.p. C. perfringens            
16 12381–14216 sim. trsK, L. lactis            X
17 14265–16145 unidentified            
24 18442–18942 unidentified            
25 18975–20306 sim. pX01 ORF-59, B. anthracis            X
28 21387–22628 unidentified           X X
29 22897–24546 unidentified            
30 24561–25625 unidentified            
32 26752–27000 unidentified           X X
33 27515–28045 unidentified           X X
35 29882–30571 sim. rep63A, AWO6 pAW63            
37 31610–32386 sim. pAW63            X
38 32577–34115 sim. repS, AWO6 pAW63           X X
39 35021–35887 sim. repB, AWO6 pAW63            
42 37951–39510 sim. S-layer precursor, B. anthracis            
44 40988–41308 unidentified            
45 41900–42211 conserved domain, several bacteria            
46 42260–42925 CAAX amino term. protease family          X X X
47 43636–44100 c.h.p. several bacteria      X   X     
48 44477–45067 transcriptional repressor, tetR fam.   X X X X   X     
49 45891–46361 IS 231            
50 46400–46891 IS 231            
51 47474–47641 sim. bacitracin            
53 49418–50866 sim. to acpA, B. anthracis            
55 52795–54195 dep            
56 54378–55612 capA            
57 55625–56074 capC            
58 56089–57483 capB            
59 60856–61407 signal peptide            
60 61759–62496 unidentified            
61 62841–63251 sim. pX01 atxA, B. anthracis            
64 68909–70360 acpA            
66 73500–75059 traC           X X
68 76097–76690 unidentified           X X
69 76918–78183 uvx           X  
71 79219–80772 unidentified            
73 82311–83936 repressor            
74 85420–85857 unidentified            
76 86664–87491 topoisomerase           X X
77 87888–88688 unidentified            
80 90752–91735 unidentified            
81 91802–93571 unidentified            X
   Column Totals 0 1 1 1 2 0 2 0 1 10 16
  1. Bacterial isolates designations are abbreviated as follows: S2-8, B. cereus S2-8; 3A, B. cereus 3A; DC-17, B. cereus DC-17; ALH, B. thuringiensis Al-Hakam; konku, B. thuringiensis subsp. konkukian; HD571, B. cereus (HRRL HD-571; F1-15, B. cereus F1-15; 4342, B. cereus ATCC 4342; 43881, B. cereus ATCC 43881; 33679, B. thuringiensis ATCC 33679; AWO6, B. thuringiensis AWO6. See Table 1 for source and Jaccard distances of AFLP profiles. 'sim.' = similar to. 'c.h.p.' = conserved hypothetical protein.
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