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Table 2 PCR assay results using primer sets designed for pXO2 ORF sequences.

From: DNA sequence conservation between the Bacillus anthracis pXO2 plasmid and genomic sequence from closely related bacteria

ORF

Nucleotides

Gene ID or similarity

S2-8

3A

DC-17

ALH

konku

HD571

F1-15

4342

43881

33679

AWO6

5

2240–3088

unidentified

          

X

7

3449–4057

unidentified

           

9

5227–7158

sim. trsE, S. aureus

         

X

X

10

7178–7846

unidentified

          

X

14

8704–11562

c.h.p. C. perfringens

          

X

15

11588–12379

c.h.p. C. perfringens

           

16

12381–14216

sim. trsK, L. lactis

          

X

17

14265–16145

unidentified

           

24

18442–18942

unidentified

           

25

18975–20306

sim. pX01 ORF-59, B. anthracis

          

X

28

21387–22628

unidentified

         

X

X

29

22897–24546

unidentified

           

30

24561–25625

unidentified

           

32

26752–27000

unidentified

         

X

X

33

27515–28045

unidentified

         

X

X

35

29882–30571

sim. rep63A, AWO6 pAW63

           

37

31610–32386

sim. pAW63

          

X

38

32577–34115

sim. repS, AWO6 pAW63

         

X

X

39

35021–35887

sim. repB, AWO6 pAW63

           

42

37951–39510

sim. S-layer precursor, B. anthracis

           

44

40988–41308

unidentified

           

45

41900–42211

conserved domain, several bacteria

           

46

42260–42925

CAAX amino term. protease family

        

X

X

X

47

43636–44100

c.h.p. several bacteria

    

X

 

X

    

48

44477–45067

transcriptional repressor, tetR fam.

 

X

X

X

X

 

X

    

49

45891–46361

IS 231

           

50

46400–46891

IS 231

           

51

47474–47641

sim. bacitracin

           

53

49418–50866

sim. to acpA, B. anthracis

           

55

52795–54195

dep

           

56

54378–55612

capA

           

57

55625–56074

capC

           

58

56089–57483

capB

           

59

60856–61407

signal peptide

           

60

61759–62496

unidentified

           

61

62841–63251

sim. pX01 atxA, B. anthracis

           

64

68909–70360

acpA

           

66

73500–75059

traC

         

X

X

68

76097–76690

unidentified

         

X

X

69

76918–78183

uvx

         

X

 

71

79219–80772

unidentified

           

73

82311–83936

repressor

           

74

85420–85857

unidentified

           

76

86664–87491

topoisomerase

         

X

X

77

87888–88688

unidentified

           

80

90752–91735

unidentified

           

81

91802–93571

unidentified

          

X

  

Column Totals

0

1

1

1

2

0

2

0

1

10

16

  1. Bacterial isolates designations are abbreviated as follows: S2-8, B. cereus S2-8; 3A, B. cereus 3A; DC-17, B. cereus DC-17; ALH, B. thuringiensis Al-Hakam; konku, B. thuringiensis subsp. konkukian; HD571, B. cereus (HRRL HD-571; F1-15, B. cereus F1-15; 4342, B. cereus ATCC 4342; 43881, B. cereus ATCC 43881; 33679, B. thuringiensis ATCC 33679; AWO6, B. thuringiensis AWO6. See Table 1 for source and Jaccard distances of AFLP profiles. 'sim.' = similar to. 'c.h.p.' = conserved hypothetical protein.
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BMC Genomics

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