Figure 3

Hybridization intensity from oligo(dT) probe, as a function of the length of the clone insert for different microarray spots Oligo(dT) was end labeled with T4 kinase, then hybridized to a microarray. The complete sequences of 18 clone inserts were then determined, corresponding to microarray spots that had a signal of >24000 phosphorimager units with a muscle cDNA probe (see Additional File 1). The lengths of the insert sequences are plotted here versus the hybridization intensity from the oligo(dT) probe.