Figure 5
From: Outliers involving the Poly(A) effect among highly-expressed genes in microarrays
Processing of images to obtain quantitative estimates of microarray spot hybridization intensities. (a) Original phosphorimager image of the radioactivity in one of the array's sixteen 12 column × 30 row matrices of spots. (b) Subtraction of background from the array image, as described in Methods. (c) Image synthesized by constructing two-dimensional gaussian functions with locations, amplitudes, and standard deviations that were estimated by fitting the spots in the image (b).