Identification of TOX subfamily predicted proteins in pufferfish and mosquito. Shown is an amino acid comparison of the TOX NLS and HMG-box regions with predicted proteins encoded in the Fugu rubripes or Anopheles gambiae genomes. Fugu rubripes predicted proteins are designated here simply by the scaffold (Scaf) location of the corresponding gene (see Methods). Amino acids that differ from the TOX HMG-box are highlighted in red, the putative NLS regions are highlighted in green, and the AXXW motif is highlighted in blue. The vertical line represents the position of the conserved exon boundary of the respective genes. Alternative amino acids found in other mammalian family members are also shown (family).