Conservation and shared usage of a polyadenylation signal sequence by mammalian ABHD1 and Sec12 cDNAs. The stop codons of ABHD1 transcripts are shown in purple. The 3'UTRs of human, Homo sapiens (Hs); mouse, Mus musculus (Mm); rat, Rattus norvegicus (Rn); cow, Bos taurus (Bt) and pig, Sus scrofa (Ss) ABHD1 and Sec12 cDNAs were aligned. For clarity, the complementary and reversed Sec12 sequences are shown. The ABHD1 polyadenylation signal is ATTAAA, coloured blue and the Sec12 signal is AATAAA (TTTATT, coloured red). The polyadenylic acid tails are shown in green. The accession numbers are: HsABHD1, AY033290; HsSec12, AF203687; MmSec12, AF150808 (all this study); Mm ABHD1, AF189764 ; RnSec12 cDNA, BI300647; RnSec12, EST BE100313; BtABHD1, EST BI541180 and SsSec12 EST, AW785453. The conserved nucleotides are indicated (*).