Expression of ABHD1 and Sec12 mRNAs in human adult tissues by semiquantitative real time PCR. (A) Expression levels in each tissue cDNA were normalised to the expression levels of the housekeeping gene β-actin. The ratios of ABHD1 and Sec12 mRNA/β-actin mRNA (Y axis, arbitrary units) from each tissue were standardised to that of Sec12 expression in skeletal muscle, which was taken as 100. (B) Ethidium bromide stained agarose gel of PCR products after 40 cycles of amplification. The tissues examined were: heart, He; whole brain, Br; placenta, Pl; lung, Lu; liver, Li, skeletal muscle, SM, kidney, Ki, pancreas, Pa; spleen, Sp; thymus, Th; prostate, Pr, testis, Te, ovary, Ov, small intestine, SI, colon, Co; peripheral blood leukocyte, Le; and no cDNA control, -c; 100 bp ladder, m.