Figure 7
Expression of ABHD1 and Sec12 mRNAs in human cell types by semiquantitative real time PCR. (A) Expression levels in each cell type cDNA were normalised to the expression levels of the housekeeping gene β-actin. The ratios of ABHD1 and Sec12 mRNA/β-actin mRNA (Y axis, arbitrary units) from each cell type were standardised to that of Sec12 expression in pulmonary adult fibroblasts, which was taken as 100. (B) Ethidium bromide stained agarose gel of PCR products after 40 cycles of amplification. The lanes are: 1, phiX174 DNA/HaeIII markers (m); 2, pulmonary artery smooth muscle (SM); 3, bronchial smooth muscle (SM); 4, HFL-1 (pulmonary foetal fibroblasts) (Fb); 5, pulmonary adult fibroblasts (Fb); 6, placental microvascular endothelial (En); 7, umbilical vein endothelial (En); 8, A549 (adenocarcinoma alveolar epithelial) (Ep); 9, H322 (adenocarcinoma bronchial epithelial) (Ep); 10, HEL (erythroleukemia) (Er); 11, K562 (erythroleukemia) (Er); 12, negative control (-c).