Figure 6

Unspecific hybridisation to similar array elements. PCR products from S. cerevisiae genes with various sequence similarities to S. pombe genes were used as array elements and hybridised with S. pombe samples. Hybridisation data are shown for 13 such genes showing 51%-79% identity to S. pombe genes across their entire lengths of ~200 bp. Blue bars: fluorescence signals (local background subtracted) picked up by the S. cerevisiae array elements; the means and standard deviations of four signal measurements are shown (one self-self experiment with two replicate measurements of both Cy3 and Cy5 each). Yellow bars: relative amount (in percentages) of 'unspecific' signals from S. cerevisiae array elements compared to 'specific' signals from the corresponding S. pombe array elements. Array elements for the following S. cerevisiae genes were used (increasing similarity; corresponding S. pombe genes in parentheses): HDA1 (SPAC8C9.06c); RPL18A (rpl18-1); CDC2 (cdc6); CDC19 (pyk1); RPL18A (rpl18-2); URA7 (SPAC10F6.03c); RPL27A (rpl27-2); HOG1 (sty1); YPT1 (ypt2); ACT1 (act1); HTA1 (hta1); HTB1 (htb1); ACT1 (act1).