Molecular cloning, genomic characterization and over-expression of a novel gene, XRRA1, identified from human colorectal cancer cell HCT116Clone2_XRR and macaque testis

Mesak, Felix M; Osada, Naoki; Hashimoto, Katsuyuki; Liu, Qing Y; Ng, Cheng E

doi:10.1186/1471-2164-4-32

Table 3 Exon-intron junctions of human (Hs) XRRA1, murine (Mm) Xrra1 and rat (Rn) Xrra1

From: Molecular cloning, genomic characterization and over-expression of a novel gene, XRRA1, identified from human colorectal cancer cell HCT116^Clone2_XRR and macaque testis

Exon No.		Exon Size (bp)	5' Splice Donor	Intron No.	Intron Size (bp)^a	3' Splice Acceptor
1	Hs XRRA1	128	GGCTCAGGAGgt aaagccataa			ttgcactcacag GGAGGCATCT
	Mm Xrra1	133	GGCTCAGGAGgt attgtcacag			ctgtgcccacag GGAGGCATCC
	Rn Xrra1	133	GGCTCAGGAGgt attatcacag			ctgtgcccacag GGAGGCATCT
2	Hs XRRA1	219	GACCGGACAGgt gagtgacacc	1	687	ccttaattgaag ACTGAAGAAG
	Mm Xrra1	206	GACCGCACAGgt gagctatggc		685	ccttaattgaag ACTGAAGAAG
	Rn Xrra1	206	GACCGCACAGgt gagcccgccc		-	tcttaattgaag ACTGAAGAAG
3	Hs XRRA1	43	AACCTCAGAGgt aggaactgac	2	43215	cttctgtttcag AGGTTGTGTT
	Mm Xrra1	44	AACCTCAGAGgt aggaggacct		4118	tttctgtttcag AGGTTGTGTT
	Rn Xrra1	44	AACCTCAGAGgt aggaggaacc		-	tttatgtttcag AGGTTGTGTT
4	Hs XRRA1	127	CTACAACAAGgt gactttctgc	3	3696	cttctttctaag ACAACCAAGA
	Mm Xrra1	126	CTACAACAAGgt aactgctttg		3706	tttcctctgaag ACAGCCAAGG
	Rn Xrra1	126	CTACAACAAGgt aaactgcttt		-	ttccctcctaag ACAACCAAGG
5	Hs XRRA1	96	CATACACGAGgt atagtgcccg	4	7073	cattcattagag ATCGCAAAAG
	Mm Xrra1	94	CACACACGAGgt atggtggcca		4393	cattcattagag ATCGCAAAAG
	Rn Xrra1	94	CACACACGAGgt atggtggcca		-	tgcactttccag ATCGCAAAGG
6	Hs XRRA1	123	ATCATGGAAGgt agggcttcca	5	779	tcatggcaccag GGGTCCCTCC
	Mm Xrra1	116	GTCACGGAAGgt aagccttcca		736	ccttcataccag GGATCCACAC
	Rn Xrra1	116	ATCGCGGAAGgt aagccttcca		-	ccctcacaccag GGATCCCACC
7	Hs XRRA1	351	CCTGACCCAGgt acctgtatcc	6	2622	tctccatgacag GTGAAAAGCG
	Mm Xrra1	324	CCTGACCCAGgt gacagccccc		2256	tctctcatccag GTGAAGACCT
	Rn Xrra1	324	CCTGACCCAGgt gacagccccc		-	tctctcatacag GTGAAAACCT
8	Hs XRRA1	202	GAGCCAAAGGgt atgtgagggc	7	2839	tcctgggcacag GTGAGTGAAC
	Mm Xrra1	196	GAGCCAAAGGgt atgggattaa		1421	ttcctggcacag GTGAATGAGC
	Rn Xrra1	199	GAGCCAAAGGgt acgggattaa		-	ttcctggcacag GTGAATGAGC
9	Hs XRRA1	117	AGAGAAACGGgt aaacatccag	8	788	ttcttgccctag GGAATCCAGA
	Mm Xrra1	116	GGAGAAGAGGgt aagcacttca		594	tgcatcctctag GCATCCAGAA
	Rn Xrra1	116	AGAGAAGAGGgt aagcacttca		-	tgcatcctccag GCATCCAGAA
10	Hs XRRA1	108	GCTCCACTAGgt acggctctgc	9	207	accttctgccag GCCCAGAGAA
	Mm Xrra1	106	GCTCCACTGGgt atggtgccag		169	tgcatcctctag GCCGGGAGGA
	Rn Xrra1	106	GCTCCACTGGgt atggtgccag		-	cgtttgtgccag GTTGCGAGGA
11	Hs XRRA1	484		10	397	cttttcctgcag GTGCTGTCCT
	Mm Xrra1	445			294	tcattcctgtag GCACTGTGTT
	Rn Xrra1	445			-	tcatccctgtag GCGCTGTGTG

^aThe intron sizes of Rn Xrra1 gene could not be determined since the sequencing of rat genomic clone CH230-188L13 (GenBank AC127923) containing the gene was in progress. Nevertheless, their splicing sites can be identified, thus the exon sizes are also known.

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ISSN: 1471-2164

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