Figure 2

Neighbour-joining tree and introns positions of A. gambiae GSTs. The putative amino acid sequences of all 32 GST subunits in A. gambiae were aligned with GST-1 from C. elegans (Accession number CAA78471) using ClustalW (see text for details). The tree was constructed by the neighbour-joining method from a similarity matrix of pairwise comparisons made using the Jukes-Cantor algorithm. Nodes with distance bootstrap values (500 replicates) of > 70% are marked by *. The positions and phase of introns are shown on the right of the dendogram. Phase 0 introns are shown by a solid black line, phase 1 by dotted lines and phase 2 by grey solid lines.