Table 2 Quantitative PCR results of A. gambiae epsilon GST genes. The transcript copy number or gene copy number was determined using cDNA or gDNA respectively. The copy numbers were normalised for variations in initial template concentration by dividing each sample by the copy number of the ribosomal protein gene, S7. The final column shows the ratio of the transcript or gene copy number between the resistant ZAN/U and insecticide susceptible Kisumu strain. Statistically significant differences (*p < 0.05, **p < 0.01) between ZAN/U and Kisumu are indicated.
Gene | Kisumu Strain Normalised cDNA copy number × 102(± S.D.) | ZAN/U Strain Normalised cDNA copy number × 102(± S.D.) | Ratio of Copy Number (ZAN/U:KISUMU) |
|---|---|---|---|
GSTe1 | 1.50 ± 0.320 | 4.52 ± 1.054 | 3.0 ** |
GSTe2 | 1.66 ± 0.408 | 12.92 ± 4.125 | 7.8 ** |
GSTe3 | 0.15 ± 0.100 | 0.49 ± 0.133 | 3.3 * |
GSTe4 | 1.13 ± 0.299 | 2.82 ± 0.795 | 2.5 * |
GSTe5 | 0.08 ± 0.050 | 0.10 ± 0.065 | 1.3 |
GSTe6 | 0.58 ± 0.223 | 1.20 ± 0.551 | 2.1 |
GSTe7 | 0.03 ± 0.010 | 0.34 ± 0.152 | 11.0 ** |
GSTe8 | 0.48 ± 0.162 | 0.62 ± 0.541 | 1.3 |
|  | Kisumu strain Normalised gDNA copy number. (± S.D.) | ZAN/U strain Normalised gDNA copy number. (± S.D. | Ration of Copy Number (ZAN/U:Kisumu) |
GSTe1 | 0.29 ± 0.125 | 0.32 ± 0.092 | 1.1 |
GSTe3 | 0.32 ± 0.088 | 0.51 ± 0.144 | 1.6 ** |
GSTe5 | 0.22 ± 0.129 | 0.29 ± 0.073 | 1.3 |
GSTe7 | 0.28 ± 0.098 | 0.21 ± 0.081 | 0.75 |