Figure 6From: Microarray analysis of tumor necrosis factor α induced gene expression in U373 human glioblastoma cellsMCP-1 ELISA analysis of supernatants of U373 cells. Supernatants of cultured U373 cells treated as indicated were analyzed with ELISA (Chemicon). Data are shown as mean + SEM of four replicates. Detection limit was 15 pg/ml human MCP-1. As compared with untreated cells (control) TNF stimulation resulted in high amounts of secreted MCP-1. PDTC blocks this induction of MCP-1 to a level below the detection limit of the assay (<15 pg/ml).Back to article page