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Table 2 Haploid DNA copy number in IMR-32 and SK-N-SH compared to a normal human control sample and fold expression difference between IMR-32 and SK-N-SH (tester vs. driver): Real-time quantitative PCR based determination of gene copy number and fold-expression of 5 previously reported amplified genes (MYCN, DDX1, NAG, NSE1, MEIS1) and 6 other amplified 2p clones (rounded mean of 2 measurements) (*primers designed in 2p24.3; **no expression in SK-S-SH).

From: Combined subtractive cDNA cloning and array CGH: an efficient approach for identification of overexpressed genes in DNA amplicons

GENE CLONE ACCESSION NUMBER LOCATION HAPLOID COPY NUMBER IN IMR-32 HAPLOID COPY NUMBER IN SK-N-SH FOLD OVER-EXPRESSION
MYCN   NM_005378 2p24.3 53 1.26 513
DDX1   NM_004939 2p24.3 42 1.28 40
NAG   AF388385 2p24.3 41 1.31 37
NSE1 g10e3 CD664581 2p24.3 70 1.27 280
  g9d9 CD664576   44 1.05 193
   NM_145175   34 0.93 236
- g10d12 CD664582 2p24.3 27 1.17 1616
TEM8 g1h7 CD664531 2p13.3 26 1.02 8
- g4d5 CD664538 2p14 30 1.04 1819
MEIS1 g6f6 CD664530 2p14 42 1.27 10
- g2h10a CD664534 2p24.3* 42 1.07 **