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Table 2 By-Gene medians and median absolute deviation

From: Triple-target microarray experiments: a novel experimental strategy

 

Triple-self hybridisations Median (MAD)

Self-self hybridisations Median (MAD)

Cy5 / Cy3

0.024 (0.141)

0.0153 (0.0943)

Cy5 / Alexa594

0.013 (0.170)

0.0066 (0.1609)

Cy3 / Alexa594

-0.001 (0.145)

0.0068 (0.1486)

  1. For each gene on the array, the median and median absolute deviation (MAD) of a particular log-ratio (e.g. Cy5/Cy3) across the 5 arrays * 2 spot replicates per array (effective n = 10) was obtained. The median value of all genes' median log-ratios and all genes' MAD is the basis of the estimate in this table. The effective n for the self-self hybridisations was 2 arrays per dye-label combination * 2 spot replicates per array = 4 measurements.