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Figure 3 | BMC Genomics

Figure 3

From: Universal Reference RNA as a standard for microarray experiments

Figure 3

Unique genes contributed by individual cell lines to UHRR. Total RNA isolated from 10 individual human cell lines were reverse-transcribed to cDNA, labeled with Cy5 and co-hybridized with Cy3-labeled UHRR onto 43,000-spot cDNA microarrays (Stanford University). The data was analyzed using GeneTraffic software. Approximately 6000–8000 spots out of 43,000 (14–18%) were flagged on each microarray and excluded from further analysis. Spots with hybridization signals in Cy5 channel higher than 1000 and with Cy5/Cy3 ratio greater than 2 were collected and the number of spots with these characteristics on only one microarray was determined.

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