Figure 4

Northern analysis Northern blot expression pattern of A. nidulans mRNAs corresponding to the cDNAs encoding (from top to bottom) phosphoenolpyruvate carboxykinase (PEPCK), heat shock protein, protein with an unknown function, endoglucanase, exopolygalacturonase, xylanase, xylose reductase, and cell wall galactomannoprotein. Total RNA was isolated from fungal mycelia and ~10 μg of RNA was separated on a 1% agarose/formaldehyde gel, blotted on nylon membranes, UV cross crosslinked and hybridized to cDNA probes as indicated. The order of total RNA in the gel from left to right are: glucose plus (GLC), glucose minus or starved (STV), rhamnogalacturonan (RHG), xylan (XYL), arabinogalactan protein (AGP), carboxy methyl cellulose (CMC), arabinan (ARA), locust bean gum (LBG), pectic galactan (PEG), gum arabic (GAR), pectin (PEC), arabinoxylan (ARX), karaya gum (KAG), xyloglucan (XYG).