Skip to main content
Figure 1 | BMC Genomics

Figure 1

From: Rapid prefractionation of complex protein lysates with centrifugal membrane adsorber units improves the resolving power of 2D-PAGE-based proteome analysis

Figure 1

Practical and theoretical 2D-PAGE analysis of total S. pombe cell lysate. (A) 0.8 mg total S. pombe protein lysate was separated by 2D-PAGE as described in Methods. The gel was stained with SYPRO Ruby and analyzed with the Phoretix 2D imaging software. (B) All proteins in the Sanger Centre S. pombe genome database with pI values between 3.0 and 10.0 possessing a molecular weight below 200 kDa were blotted in the diagram to indicate a virtual whole cell 2D-PAGE.

Back to article page

BMC Genomics

ISSN: 1471-2164

Contact us