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Figure 2 | BMC Genomics

Figure 2

From: Rapid prefractionation of complex protein lysates with centrifugal membrane adsorber units improves the resolving power of 2D-PAGE-based proteome analysis

Figure 2

Sample prefractionation with Vivapure Q Mini spin columns (A) 4 mg total fission yeast protein was loaded onto a Vivapure Q mini spin column, washed, and eluted with a NaCl step gradient from 50 mM to 1 M. The elution profile is shown. (B) Summary of the binding and elution characteristics of Vivapure Q Mini columns. (C) Analysis of fractions by 1D PAGE. The flowthrough as well as low and high salt fractions showed different protein patterns.

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BMC Genomics

ISSN: 1471-2164

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