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Table 1 Amplification factors

From: Systematic analysis of T7 RNA polymerase based in vitro linear RNA amplification for use in microarray experiments

tot RNA starting amount [ng]

Repetitions

Range of amplified RNA [μg]

Average amount of amplified RNA [μg]

Range of amplification factors

Average amplification factor*

100–200

14

1–4.4

2.49

200–560

368

1,000–3,200

16

12–36

22

150–500

253

  1. * calculated under the assumption that 5% of total RNA corresponds to poly (A)+ RNA
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BMC Genomics

ISSN: 1471-2164

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