Multi-species alignment of ELN proteins from eight mammalian species. Amino acids with similar chemical characteristics are color-coded (see notes below). Human, cow, mouse, and rat are derived from GenBank sequences; baboon, cat, dog, and pig are predicted from genomic sequences based on the similarity to human and mouse ELN genes. Color legend: H, K, R – polar/positively charged amino acids; D, E – polar/negatively charged; N, Q – polar/amide; S, T – polar/alcohol; L, I, V – non-polar/aliphatic; F, Y, W – non-polar/aromatic; A, G, P – other non-polar. Domain information is shown below the alignment; alternating cross-linking (designated as white boxes) and hydrophobic (yellow boxes) domains are shown. Exon borders are marked with black arrows at the top. Grey arrows mark the beginning of exons 4A (found in rodents) and 26A (human-specific, ), respectively.