A. QRT-PCR analysis of hTERT and hTERC in primary tumor cultures compared to levels in normal breast organoids, matched fibroblasts, and immortal breast epithelial cell lines (T47D, MDA231, and ENUt7). The Y-axis is minimized to display the range of relative gene expression. The highest hTERT expression level, denoted by the asterisk, was 22-fold (sample 257T). B. TRAP assay measurement of telomerase activity shown with, and without heat inactivation of cell lysates. Extracts equivalent to 1000 cells are displayed. IC – internal PCR control. For sample 054T, 2 independent fractions of the tumor are shown; SP – mechanically dissociated spillage, DIG – enzymatically digested tissue.