Fugu genome sequence and annotation. A. Fugu selectin gene cluster annotation in the EnsEMBL database (v.2 annotation is shown, v.3 annotation is almost identical to v.2). Gene models predicted by us based on comparison with human selectins are shown in the grey box. As shown, the CTLD is encoded by the 5' exon in fSELP, fSELL and fSELE; the TM segment is encoded by the 3' exon. EnsEMBL predicted transcripts, GenScan predictions and similarity features are shown on the tracks below. Stable IDs for EnsEMBL transcripts are given. The TMHMM track shows ORFs encoding TransMembrane regions predicted by the TMHMM program (see Methods). B. Fragments of group VI genes found on various scaffolds. CTLD numbers indicate sequential number of CTLD in full-length MManR, while numbers for the CTLD in the partial sequences indicate the MManR CTLD sequence they are most closely homologous to.