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Figure 2 | BMC Genomics

Figure 2

From: Methods for high throughput validation of amplified fragment pools of BAC DNA for constructing high resolution CGH arrays

Figure 2

Use of AFPs as hybridisation probe in Southern Analysis. 200 ng RP11-156K13 Hind III digest (lane 1). 200 ng RP11-104F14 Hind III digest (lane 2). (A) In silico fingerprint of RP11-156K13 and RP11-104F14 generated from the BAC database [19] using the FPC software [20]. (B) Southern transfer hybridized with radiolabeled AFP from BAC clone RP11-156K13 without Cot-1 DNA blocking. (C) Southern transfer hybridized with radiolabeled AFP from BAC clone RP11-156K13 with 50 μg Cot1 DNA blocking.

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BMC Genomics

ISSN: 1471-2164

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