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Table 1 Comparison of three techniques for AFP identification.

From: Methods for high throughput validation of amplified fragment pools of BAC DNA for constructing high resolution CGH arrays

MERITS FISH ANALYSIS SOUTHERN ANALYSIS BAC END SEQUENCING
Chromosomal localization Genomic position No Genomic position
Verification of AFP to original BAC No Yes Yes
Representation of original BAC in AFP No Yes No
Relative cost per assay Expensive Moderately expensive Inexpensive
Time 3+ Days 3 days 4 hours
Other Requires normal cell line for metaphase Requires 200 ng of digested BAC DNA Can be automated for high throughput analysis