Figure 1

Artificial enhancer trapping with a Sleeping Beauty transposon. Comparison of GFP expression in embryos injected with pT2/S1 EF1α or γCry1/pT2/S1 EF1α. Plasmids are diagramed as cartoons on the top of the picture. The SB transposon's inverted repeats are shown as boxes with open triangles, and the GFP open reading frame is depicted as a grey arrow. The gamma-Crystallin promoter/enhancer is shown as a hatched box. DNA-injected embryos which survived to 3 dpf were counted and scored for GFP fluorescence anywhere in the embryo (any GFP) and for fluorescence in the eye (eye GFP), even if there was additional fluorescence elsewhere. The average percentage of embryos positive for particular GFP fluorescence in three independent experiments is shown ± standard deviation.