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Table 1 Process steps The process fail points for cDNA based microarray production. Steps shown in italics are accessible to the testing methods outlined here. Earlier steps may require sequencing to test. The process ID is used to identify steps where the problems, if any, are found.

From: A method for detecting and correcting feature misidentification on expression microarrays

Process ID Problem Type Note
-999 Unidentified  
0.0 Source.General persists across all arrays at clone, 96 level
0.1 Source.Contamination  
0.2 Source.MisID  
1.0 Prep.General persists across all arrays at clone, 96 level
1.1 Prep.Contamination  
1.2 Prep.MisID  
1.2.1 Prep.MisID.OrderError  
1.2.2 Prep.MisID.Rot  
1.3 Prep.Fail  
2.0 PCR.General persists for 1 PCR round at 96 level
2.1 PCR.Contamination  
2.2 PCR.MisID  
2.3 PCR.Fail  
2.4 PCR.Cleanup  
3.0 Replate.General persists for 1 PCR round at 96 level
3.1 Replate.Contamination  
3.2 Replate.MisID  
3.2.1 Replate.MisID.OrderError  
3.2.2 Replate.MisID.96Rot  
3.2.3 Replate.MisID.384Rot  
4.0 Print.General persists for 1 print run batch at 384 level
4.1 Print.Contamination  
4.2 Print.MisID  
4.2.1 Print.MisID.OrderError  
4.2.2 Print.MisID.Rot  
4.3 Print.Fail dried out plate, too concentrated, too weak etc.
5.0 Scan.General anything after array production