Figure 2

Amplifications in MYCN amplified samples. A. Regression analysis of MYCN ratio (sample vs. normal) obtained from A-CGH and real-time Q-PCR in all of neuroblastoma samples including 13 cell lines and 32 primary tumors. The slope of the regression line is 0.35 indicating that an observed A-CGH ratio of 2 is equivalent to a Q-PCR ratio of 5.7. B. Independent amplicons in chromosome 2p. All amplified genes are listed under each amplicon in genome order. Map position, genome sequence position (Mb) and samples containing the specific amplicon are listed for each amplicon. The percentage of the MYCN amplified samples harboring these amplicons are shown in brackets following the gene name for all clones present in our microarray (gray), the remainder of the clones are predicted genes found in the NCBI database http://www.ncbi.nlm.nih.gov/genome/guide/human/ that are mapped between the boundaries of the amplicon. Amplicons were selected based on the criteria of A-CGH ratio ≥2 for at least two contiguous clones in genome sequence order. In cases where a single clone has a ratio <2 but the ratio of its adjacent clones is greater than 2, that single clone was still considered as a part of amplicon. *: previously reported amplification. C. Amplicon in chromosome 12q in tumor NB21.