Analysis of aRNA generated after different cDNA purification methods. (A) Fluorescence absorptions at 100 nucleotides (100 nt), 1000 nucleotides (1000 nt), and 6000 nucleotides (6000 nt) data points. The results were obtained by plotting the mean fluorescence absorption values for the five cell lines using the different cDNA purification methods. cDNA purification methods: Col. – Column; E-R – ethanol at room temperature; IS – Isopropanol; E-C – ethanol at cold temperature. (B) Pattern of amplified RNA from OCUB-M cell line using cDNA purified by isopropanol method. First peak-absorption at about 100 nucleotides; second peak-absorption at 1000 nucleotides. (C) Pattern of amplified RNA from OCUB-M cell line using cDNA purified by ethanol at room temperature method.