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Table 1 Yield of RNA amplification generated from different cDNA purification methods.*

From: Expression microarray reproducibility is improved by optimising purification steps in RNA amplification and labelling

Method 28S/18S = 1.9 28S/18S = 1.4
column 18 μg ± 2 7 μg ± 1
ethanol at RT 17 μg ± 2 10 μg ± 1
isopropanol 19 μg ± 3 14 μg ± 2
ethanol at CT 14 μg ± 2 12 μg ± 1
  1. *RNA from MCF-7 and HCC-1187 (28S/18S = 1.9) and OCUB-M (28S/18S = 1.4) were used and reactions were repeated five times. Values represent averages with standard errors.