Comparative analysis of the kinomes of three pathogenic trypanosomatids: Leishmania major, Trypanosoma brucei and Trypanosoma cruzi

Table 1 Groups and families of ePKs in the TriTryps

Group	Family	*T. brucei*	*T. cruzi* ^a	*L. major*	Group	Family	*T. brucei*	*T. cruzi*	*L. major*
AGC	(na)^b	6	7	6	Other	AUR	3	3	3
	NDR	2	1	1		CAMKK	4	4	4
	PKA	3	3	3		CK2	2	2	2
	RSK	1	1	1		NAK	0	1	1
	Total	12	12	11		NEK	20	22	22
CAMK	(na)	7	7	9		PEK	3	2	3
	CAMKL	7	6	7		PLK	2	2	1
	total	14	13	16		TLK	2	1	1
CK1	CK1	4	7	6		ULK	1	1	1
	TTBK	1	1	1		VPS	1	1	0
	Total	5	8	7		WEE	1	2	2
CMGC	(na)	1	1	1		total	39	42	40
	CDK	11	10	11	STE	(na)	7	8	10
	CLK	4	5	4		STE7	2	2	2
	DYRK	7	7	8		STE11	14	18	21
	GSK	2	2	2		STE20	2	3	1
	CDKL (MAPK-like)^c	2	1	2		total	25	31	34
	MAPK^d	10	11	12	Unique		19	23	26
	RCK (MAPK-like)^c	3	3	3	TOTAL ePK		156	171	179
	SRPK	2	2	2
	total	42	42	45	Non-catalytic		13	16	16

^a Multiple copies of TcCRK7 were counted as one gene. The number of tandem repeated CK1 genes in T. cruzi is not clear. One putative T. cruzi PEK gene is truncated by the contig end for both alleles, and is assigned on the basis of orthology in the non-catalytic sequence.
^bna, not assigned to a family.
^c CDKL and RCK families are related to MAPKs and CDKs [57] but are classified separately in the human kinome [21].
^d One MAPK-like sequence in T. brucei and two in T. cruzi and L. major lack the typical regulatory motifs (see Table S1). One MAPK-like COG, which fell below the E-value criterion, was designated as MAPK on the basis of regulatory motifs.

ISSN: 1471-2164