Comparative analysis of the kinomes of three pathogenic trypanosomatids: Leishmania major, Trypanosoma brucei and Trypanosoma cruzi

Table 2 Additional Pfam domains on trypanosomatid ePKs

Pfam	Tb^a	Tc	Lm	TriTryp^b	Other kinomes^b	Comments
Armadillo	0	1	1	ULK	NEK, STE11, ULK	related to HEAT
B-box Zn finger	0	0	1	NEK	nd	Zn binding
C1-like	0	1	1	AGC	AGC	possible diacylglyerol binding
cap-gly	1	1	1	CAMK	VPS15	cytoskeleton associated
cNMP binding	0	1	2	STE	AGC	cyclic nucleotide binding
EF Hand	2	2	2	CAMK	CAMK	Calcium binding
FHA	1	2	4	CAMK, STE	CAMK	phosphopeptide binding
FYVE	1	2	1	AGC	nd	Zn binding
HAMP	0	1	0	STE	nd	in diverse signaling proteins
HEAT	1	1	0	ULK	VPS15	protein interaction
HECT	0	0	1	unique	nd	ubiquitin transferase
Kelch	1	0	0	unique	nd	propeller structure
LRR	1	1	1	CAMKK	TK, TKL, plantrk	protein interaction
MORN	1	1	1	STE	TKL, unique	unknown function
PAS	1	1	1	STE	CAMK, TKL	signal sensor
PH	6	7	6	AGC, NEK	AGC, CAMK, STE20	Phosphatidylinositol binding
PKC-Cterm	0	0	2	AGC	AGC	found on protein kinases
POLO	1	1	1	POLO	POLO	POLO kinase region
PX	1	1	1	AGC	AGC	phosphoinositide binding
RWD	1	0	0	PEK	PEK	unknown function
TPR2	0	2	1	STE, unique	plantrk	protein interaction
zf-C2H2	2	1	1	unique	AUR	zinc binding

^a The number of genes bearing the indicated Pfam domain.
^b Classification of ePKs bearing the designated domain in the TriTryps or other kinomes (as detected in S. cerevisiae, Schizosaccharomyces pombe, P. falciparum, C. elegans, D. melanogaster, A. thaliana, and Oryza sativa) using the web tools at the Kinases in Genomes (KING) website [81]. The plantrk group is comprised of serine/threonine receptor kinases present in plant; nd, none detected.

ISSN: 1471-2164