Comparative map of the annotated BAC contig with its syntenic region on Hsap3q21-q22. The contig is drawn in part C. Black triangles represent BAC end sequences from which primers were designed to construct the contig. The black circle represents the only internal BAC sequence from which primers were designed to construct the contig. White circles show overlaps of these BAC sequences with other BAC clones. White triangles represent BESs from which it was impossible to design primers. The triangles point towards the 3'-side of the BAC clone. Encircled triangles represent BAC ends that are also present in the Sanger framework map. Black diamonds represent microsatellite positions. Black squares represent genes annotated by PCR, whereas white squares represent genes annotated by hybridization. Annotated sequences (genes are in regular, ESTs in italic) from the BAC contig are represented on a plane map (B) and their homology with the human genome sequence is illustrated with dotted lines (A). The orientation of the human genes and finished HTGs (used to assemble the human genome sequence) are represented in (A) by arrows . Repeat sequences (white rectangle = LINE, black rectangle = SINE, black bow = LTR element, white bow = DNA element) are shown on a plane map in D. The RH mapping results are shown in E.